| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | All gels were pre-cast from 4–20% “ExpressPlus PAGE Gels” from “GenScript.” A “Qubit 2.0 Fluoremeter” instrument as detailed previously [33] was used to equalize loadings in each gel. They were run at 140 volts for about 70 minutes in Tris-MOPS (SDS) running Buffer also from “GenScript.” Protein Ladders used were either “BLUEstain” Protein Ladder from “Gold Biotechnology, Inc. (St. Louis, MO) or “Precision Plus Protein “WesternC” Standards (from Bio-Rad, Hercules, CA). | Get A Quote |
In the previous paper, we reconstructed the entire transcriptional network for all 2418 clockassociated genes in the model filamentous fungus, Neurospora crassa (N. crassa). Several authors have suggested that there is extensive post-transcriptional control in the genome-wide clock network (IEEE 3: 27, 2015). Here we have successfully reconstructed the entire clock network in N. crassa with a variable topology ensemble method (VTENS), assigning each clock-associated gene to the regulation of one or more of five transcription factors as well as to six RNA operons. The resulting network provides a unifying framework to explore the clock's linkage to metabolism through post-transcriptional regulation, in which ~85... More
