| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | All chemicals were of analytical grade and used without further purification. Glycidyl methacrylate (GMA), acetonitrile (ACN), ethylene dimethacrylate (EDMA), 1-propanol, 1,4-butanediol, 3- methacryloxypropyl-trimethoxysilane (MAPS), aminomethyl phosphonic acid (AMPA), ammonium hydroxide (NH4OH), glutaraldehyde, ammonium hydrogen carbonate (NH4HCO3), acetone, calcium chloride (CaCl2), pyridine, trifluoroacetic acid (TFA), tetrahydrofuran (THF), trypsin, β-casein, cytochrome-c and polystyrene standard set (Mw 500-2,000,000) were purchased from Sigma-Aldrich (Singapore). Titanium tetrachloride (TiCl4) were obtained from Tokyo Chemical Industries, Co. Ltd (Chuo-ku, Japan). Tyrosine phosphorylated peptides sequence TSTEPQ[pY]QPGENL were purchased from GenScript (Carlsbad, USA). Acetone, ethanol, sodium chloride (NaCl), hydrochloric acid (HCl), sodium hydroxide (NaOH), and toluene were from Merck KGaA (Darmstadt, Germany). Silicon steel column (1.02 mm i.d.1/16 inch o.d) and 2,2’-azobisisobutyronitrile (AIBN) were obtained from Supelco (Bellefonte, Pennsylvania, USA) and Himedia (Mumbai, India), respectively. | Get A Quote |
Phosphopeptides present in a large number of biological regulating mechanisms. It plays a significant role in gene regulation, eukaryotic signal transduction, and metabolic control in a cell. Abnormal phosphorylation can cause various diseases, including cancer. However, phosphoprotein abundances are very low, only 1-2%. In this work, the nanoporous monolith columns were developed for separation and detection of phosphopeptides in phosphoproteome analysis. The nanoporous monolith-based column is prepared inside a silicosteel tubing (100 x 1.02 mm i.d.) by insitu copolymerization reaction of glycidyl methacrylate (GMA) with ethylene dimethacrylate (EDMA). This monolith was further chemically modified by introduc... More
