| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | High-purity chitosan (CHI) with a degree of deacetylation: viscosity of 85:2500, 95:2500, 85:1000 was purchased from Heppe Medical Chitosan GmbH (Halle, Germany). Cellulose nanocrystals (CNCs) (rod shaped; degree of polymerization 137; half-sulfate ester 254 mmol/kg CNCs; diameter 4−5 nm and length 300−400 nm) were provided by FPInnovations (Pointe-Claire, QC, Canada). Monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) was procured from Genscript USA Inc. (Piscataway, NJ, USA). Horseradish peroxidase (HRP) conjugated polyclonal anti-mouse secondary antibody (pAnti-mSec-Ab) was purchased from Jackson ImmunoResearch (Baltimore, PA, USA). Glycerol (GLY) and sulfuric acid (98 % purity) were from Laboratoire Mat (Beauport, QC, Canada). Sodium tripolyphosphate (TPP) and glutaraldehyde (GA) solution were from Sigma-Aldrich (Mississauga, ON, Canada). | Get A Quote |
This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal ... More
