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| Peptide Synthesis | The sequences were compared to known sequences using the BLAST program available on the National Center for Biotechnology Information (NCBI) website (http://www.ncbi.nlm.nih.gov/blast). The ORF was analyzed using ORFfinder (https://www.ncbi.nlm.nih.gov/orffinder/). The multiple sequence alignments were performed by DNAMAN 8.0 software (Lynnon BioSoft). The molecular weight, theoretical pI and amino acid composition of the putative proteins were analyzed with ProtParam (http://www.expasy.org/tools/protparam.html). The potential signal peptide was predicted using the SignalP 4.1 Server (http://www.cbs.dtu.dk/services/SignalP/) and the transmembrane region was predicted using the TMHMM (http://www.cbs.dtu.dk/services/TMHMM/). The 3D structure was predicted by the Swiss‐model (http://swissmodel.expasy.org/tools) and the subcellular localization was predicted by the WoLFPSORT (https://www.genscript.com/wolf-psort.html). The phylogenetic tree was constructed by Mega 7.0 using the maximum likelihood (ML) method with a total of 1000 bootstrap replicates performed (Kumar et al. 2016). | Get A Quote |
Ulva prolifera is a green‐tide‐forming macroalga dominating the green tides in the Yellow Sea from 2007 to 2019. The exponential growth of U. prolifera, and the correlating accumulation of biomass, rely heavily on its carbon fixation capability. Despite the importance of carbon fixation in algal growth, to date none of the genes involved in carbon fixation have undergone molecular characterization in U. prolifera. This study used Rapid Amplification of cDNA Ends (RACE) to characterize one full‐length carbonic anhydrase (CA) gene of U. prolifera (UpαCA1). An activity assay showed that UpαCA1 was an active CA. Real‐time quantitative PCR results showed that temperature, irradiance, salinity and pH c... More
