| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … Louis, MO, USA) with bovine serum albumin as a standard Anti-Myc monoclonal antibodies were prepared by the GenScript Company (Nanjing, China) and used to measure Myc tagged MdALMT14 protein levels for western-blot analysis … |
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Heavy metal contamination is a major environmental and human health hazard in many areas of the world. Organic acids sequester heavy metals and protect plant roots from the effects of toxicity; however, it is largely unknown how these acids are regulated in response to heavy metal stress. Here, protein kinase MdSOS2L1 from apple was functionally characterized. MdSOS2L1 was found to involve in the regulation of malate excretion, and inhibit cadmium uptake into roots. Using the DUAL membrane system in a screen of an apple cDNA library with MdSOS2L1 as a bait, a malate transporter, MdALMT14, was identified as an interactor. Bimolecular fluorescence complementation, pull-down and co-immunoprecipitation assays furth... More
