Kif20 (Kinesin Family Member 20A), plays a role in cell mitosis, cell migration and intracellular transport. Numerous studies have demonstrated that Kif20a is abnormally highly expressed in a variety of tumors and shows poor prognosis. Soft tissue sarcoma (STS) represents a group of malignant tumors with poor prognosis. The role of Kif20 in STSs has not been systematically studied. In the present study, bioinformatics analysis, and experiments were conducted to investigate the function of Kif20 in STSs. In bioinformatics analysis higher expression indicated a poor prognosis. Functional enrichment analysis indicated that may be related to cell cycle, p53 and other signaling pathways. experiments showed that after the down-regulation of , cell proliferation, migration and invasion were decreased, while apoptosis was increased. experiments revealed that affected the proliferation of tumors in tumor-bearing mice. In summary, our findings revealed that performs an important role in STS, indicating that it is a potential prognostic biomarker and potentially representing a therapeutic target for the disease.