| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … optimized TMCO1 genes from different species (Table S1) were synthesized by GenScript Biotech Company (Nanjing) and amplified by polymerase chain reaction (PCR) extension with Q5 polymerase (30 cycles of 30 s at 95 o C, 30 s at 55 o C, 30 s at 72 o C). The genes were … | Get A Quote |
Transmembrane and coiled-coil domains 1 (TMCO1) has a highly conserved amino acid sequence among species, indicating a critical role of TMCO1 in cell physiology. The deficiency of TMCO1 in humans is associated with cerebrofaciothoracic dysplasia (CFTD), glaucoma, osteogenesis and the occurrence of cancer. TMCO1 was recently identified as an endoplasmic reticulum (ER) Ca load-activated Ca (CLAC) release channel, which prevents ER Ca overload and maintains calcium homeostasis in the ER. However, the structural basis of the molecular function of TMCO1 channel remains elusive. To determine the structure of TMCO1, we screened the expression of TMCO1 in Escherichia coli and insect cell expression systems. TMCO1 from ... More
