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FACT and FAIR with Big Data allows objectivity in science: The view of crystallography

Struct Dyn. 2019; 
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Codon Optimization … Expression and purification of ELK1 DNA binding domain The recombinant ELK1-DBD (1-94 amino acid from PDB structure 1DUX) in pGEX-4T1 plasmid was purchased from GenScript the service provider of synthetic gene after codon optimization … Get A Quote

摘要

A publication is an important narrative of the work done and interpretations made by researchers securing a scientific discovery. As The Royal Society neatly states though, "Nullius in verba" ("Take nobody's word for it"), whereby the role of the underpinning data is paramount. Therefore, the objectivity that preserving that data within the article provides is due to readers being able to check the calculation decisions of the authors. But how to achieve full data archiving? This is the raw data archiving challenge, in size and need for correct metadata. Processed diffraction data and final derived molecular coordinates archiving in crystallography have achieved an exemplary state of the art relative to most fi... More

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