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Molecular Biology Reagents> | … Spleens from euthanized Pmel-1 mice were excised and splenocytes isolated mechanically and cultured at a concentration of 1.5×10 6 /mL for 48 hours with 100 ng/mL of human gp100 25-33 (KVPRNQDWL, RP20344, GenScript, Piscataway, New Jersey, USA) … | Get A Quote |