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Proteomic analysis reveals pathogen-derived biomarkers of acute babesiosis in erythrocytes, plasma, and urine of infected hamsters

Parasitol Res. 2020; 
Ruben Magni, Alessandra Luchini, Lance Liotta, Robert E Molestina
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Peptide Synthesis … The selection of suitable peptide sequences for immunization, synthesis of peptide antigens, and the production of affinity purified antibodies were per- formed by GenScript, Piscataway, NJ. Peptide amino acid sequences are available upon request … Get A Quote

摘要

Babesiosis among humans is on the rise in North America. Current diagnostic assays for the screening of babesiosis require blood collection by venipuncture, which is an invasive method. Urine on the other hand is a desirable biospecimen for biomarker analysis of Babesia microti infections because it can be collected periodically and non-invasively. Our group uses a new class of biomarker harvesting nanocage technology, which, when combined with mass spectrometry (MS), can determine the presence of parasite proteins shed in different bodily fluids of mammalian hosts, including urine. Using the hamster model of babesiosis, our nanoparticle-MS approach identified several B. microti proteins in erythrocytes, plasma... More

关键词

Babesiosis, Mass spectrometry, Nanoparticle, Proteomics, biomarker
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