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Combinatorial-Hierarchical DNA Library Design Using the TeselaGen DESIGN Module with j5

Methods Mol Biol. 2020; 
Michael J Fero, James K Craft, Trang Vu, Nathan J Hillson
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Proteins, Expression, Isolation and Analysis … across designs. The system optimizes assembly reactions to take advantage of a variety of DNA sourcing options including DNA synthesis vendors such as Twist, IDT, and GenScript. Important features of the DESIGN module … Get A Quote

摘要

Modern DNA assembly techniques are known for their potential to link multiple large DNA fragments together into even larger constructs in single pot reactions that are easier to automate and work more reliably than traditional cloning methods. The simplicity of the chemistry is in contrast to the increased work needed to design optimal reactions that maximize DNA fragment reuse, minimize cost, and organize thousands of potential chemical reactions. Here we examine available DNA assembly methods and describe through example, the construction of a complex but not atypical combinatorial and hierarchical library using protocols that are generated automatically with the assistance of modern synthetic biology softwar... More

关键词

Bioinformatics, Cloning, DNA assembly, Metabolic engineering, Pathway engineering, Synthetic biology
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