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Molecular basis for kinin selectivity and activation of the human bradykinin receptors

Nat Struct Mol Biol . . 2021-09; 
Yu-Ling Yin, Chenyu Ye, Fulai Zhou, Jia Wang, Dehua Yang, Wanchao Yin, Ming-Wei Wang, H Eric Xu, Yi Jiang
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Peptide Synthesis The washed membranes were re-suspended in 20 mM HEPES pH 7.4, 100 mM NaCl, 10 mM MgCl2, 5 mM CaCl2, 10% glycerol, 40 μM peptide (des-Arg10-kallidin for B1R and bradykinin for B2R, respectively, GenScript), 25 mU ml−1 apyrase (Sigma-Aldrich), 100 μM TCEP (Sigma-Aldrich)... Get A Quote

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