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Lyophilized, thermostable Spike or RBD immunogenic liposomes induce protective immunity against SARS-CoV-2 in mice

Sci Adv. 2021-12; 
Moustafa T Mabrouk, Kevin Chiem, Edurne Rujas, Wei-Chiao Huang, Dushyant Jahagirdar, Breandan Quinn, Meera Surendran Nair, Ruth H Nissly, Victoria S Cavener, Nina R Boyle, Ty A Sornberger, Suresh V Kuchipudi, Joaquin Ortega, Jean-Philippe Julien, Luis Martinez-Sobrido, Jonathan Lovell
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Proteins, Expression, Isolation and Analysis … blocked with 2% BSA in PBS containing 0.1% Tween 20 (PBS-T) for 2 hours at 37°C. Mouse sera (serially diluted 10-fold in PBS-T containing 1% BSA) were incubated in the wells for 1 hour at 37°C and then washed with PBS-T. Goat anti-mouse IgG-HRP (GenScript, catalog no … Get A Quote

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[Figure: see text].

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