background: Graves' orbitopathy (GO) is an autoimmune driven manifestation of Graves' disease (GD) where pathogenic autoantibodies to the TSH-receptor activate orbital fibroblasts/preadipocytes in the orbital tissue to induce inflammation and extracellular matrix deposition. Since there are significant limitations to study immunological and proinflammatory mediator expression in early and during disease progression in GO patients, we used our experimental mouse model to elucidate early pathogenic processes.
methods: We have developed a robust mouse model of GD/GO induced by electroporation immunization of plasmid encoding human TSHR A-subunit, comprising of multiple injections over a course of 15 weeks to fully... More
background: Graves' orbitopathy (GO) is an autoimmune driven manifestation of Graves' disease (GD) where pathogenic autoantibodies to the TSH-receptor activate orbital fibroblasts/preadipocytes in the orbital tissue to induce inflammation and extracellular matrix deposition. Since there are significant limitations to study immunological and proinflammatory mediator expression in early and during disease progression in GO patients, we used our experimental mouse model to elucidate early pathogenic processes.
methods: We have developed a robust mouse model of GD/GO induced by electroporation immunization of plasmid encoding human TSHR A-subunit, comprising of multiple injections over a course of 15 weeks to fully recapitulate the orbital pathology. In this study, we investigated kinetics of GO development in the model by serial analyses of immunological and cellular parameters during course of orbital inflammation.
results: Pathogenic anti-TSHR antibodies with thyroid stimulating properties developed early after the second immunization step with concomitant induction of hyperthyroidism. Examination of orbital tissue showed an early wave of macrophage infiltration followed subsequently by CD3+ T-cells into the orbital tissue. Examination of antigen-specific T-cell activity using recombinant human A-subunit protein showed high CD8+ T-cell proliferation during this early phase of disease onset, whereas effector CD4+ T cells and CD25+FOXP3+ regulatory T-cells were downregulated. The early phase of disease was also characterized by abundant presence of proinflammatory cytokines IFN- and TNF-. Moreover, as the disease progressed there was significant increase in browning of orbital fat tissue, which may be dependent on the pro-inflammatory milieu and/or the increased thyroid hormone levels during the established hyperthyroid status.
conclusions: This work revealed early infiltration of macrophages in the orbital region and induction of pathogenic anti-TSHR antibodies during disease onset in the model. This was followed subsequently by influx of CD8+ T cells specific for TSHR coupled with reduction in Tregs and substantial increase in BAT. These new insights into the development of orbital inflammation in the model have implications for testing new therapeutic regimes by targeting macrophage function during early phases of orbital inflammation in the model.
