| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | … After cooling, 15 μL of sample (1 μg/μL) was loaded on 10% precast gels (GenScript, Nanjing, China). A broad pre-stained protein standard (MW 5–270 kDa; GenScript, Nanjing, China) was used to estimate sample protein size. Electrophoresis was conducted at 90 V for 20 min … | Get A Quote |
Myoglobin is a protein not easily broken down by digestive enzymes due to its rigid structure. This study evaluated the structural characteristics of myoglobin under various sodium chloride treatments (0.4-0.8 mol/L for 5-10 h) and the impacts on its digestibility using spectroscopic and molecular dynamics simulation techniques. Myoglobin digestibility was 40% following pepsin digestion and 60% after being sequentially digested by pepsin and trypsin. The α-helix content of myoglobin did not change significantly following sodium chloride treatment but hydrophobic amino acids were exposed and the binding of phenylalanine targeted by some digestive enzymes became more stable, leading to the reduced digestibilit... More
