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Gene Synthesis> | … expressing the full length of TKR-1 (NCBI Reference Sequence: NP_499064.2) was created by GenScript. The expression vector, TKR-1-pcDNA3.1, was transfected into Chinese hamster ovary (CHO) cells. Thereafter, cells stably expressing TKR-1 were selected using 1 mg/ml … | Get A Quote |
Tachykinin-like peptides, such as substance P, neurokinin A, and neurokinin B, are among the earliest discovered and best-studied neuropeptide families, and research on them has contributed greatly to our understanding of the endocrine control of many physiological processes. However, there are still many orphan tachykinin receptor homologs for which cognate ligands have not yet been identified, especially in small invertebrates, such as the nematode Caenorhabditis elegans (C. elegans). We here show that the C. elegans nlp-58 gene encodes putative ligands for the orphan G protein-coupled receptor (GPCR) TKR-1, which is a worm ortholog of tachykinin receptors. We first determine, through an unbiased biochemica... More