Mycoplasma synoviae is a common pathogen affecting poultry and has important economic significance. Infectious synovitis is the most common clinical effect. Since 2010, the incidence of M. synoviae infection in China has rapidly risen, causing significant economic losses to the chicken industry; however, the cause of the disease outbreak remains unclear. Phylogenetic and evolutionary analyses of field strains will help unravel the mystery. The multi-locus sequence typing (MLST) method is typically utilized to conduct genotyping and traceability analysis of microorganisms. MLST of M. synoviae has previously been established and shown strong discriminatory power. In this study, 54 Chinese M. synoviae strains isolated from 2016 to 2020 were genotyped by MLST based on seven housekeeping genes. This study aimed to investigate the dominant genotypes of M. synoviae in China and reveal the genetic and evolutionary relationships of these isolates. All 54 isolates were found to have new allelic sequences, which may indicate new sequence types. The results of BURST analysis indicated that all 54 strains belonged to group 11, which is an independent phylogenetic branch, and were separated from any other reference strains (189 isolates) in the PubMLST database. In conclusion, the results of this study suggest that the M. synoviae strains circulating in China are relatively independent in terms of transmission and evolutionary relationships.