Pyroptosis is involved in ovulation of zebrafish
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The IL-1β recombinant protein was expressed and purified from E.coli bacterial system (Genscript, China)...A peptide (CRQNLFDEKSVTRTG) of zebrafish Nlrp1 was selected as antigen for the production of polyclonal antibody (Genscript, China). The synthetic peptides were used to immunize New Zealand white rabbits for antibody production (Genscript, China). |
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