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USP29 Deubiquitinates SETD8 and Regulates DNA Damage-Induced H4K20 Monomethylation and 53BP1 Focus Formation

Cells. 2022-08; 
Yeray Hernández-Reyes, María Cristina Paz-Cabrera, Raimundo Freire, Veronique A J Smits
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Proteins, Expression, Isolation and Analysis … Acetylation of histones usually results in changes in chromatin structure and is associated … After sonication, the extracts were incubated with Ni-NTA agarose beads (Genscript) for 2 h at … Get A Quote

摘要

SETD8 is a histone methyltransferase that plays pivotal roles in several cellular functions, including transcriptional regulation, cell cycle progression, and genome maintenance. SETD8 regulates the recruitment of 53BP1 to sites of DNA damage by controlling histone H4K20 methylation. Moreover, SETD8 levels are tightly regulated in a cell cycle-dependent manner by ubiquitin-dependent proteasomal degradation. Here, we identified ubiquitin-specific peptidase 29, USP29, as a novel regulator of SETD8. Depletion of USP29 leads to decreased SETD8 protein levels, an effect that is independent of the cell cycle. We demonstrate that SETD8 binds to USP29 in vivo, and this interaction is dependent on the catalytic activity... More

关键词

53BP1, DNA damage response, H4K20me, SetD8/Set8/PR-Set7/KMT5A, USP29, cell cycle, histone methylation
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