Human diamine oxidase cellular binding and internalization in vitro and rapid clearance in vivo are not mediated by N-glycans but by heparan sulfate proteoglycan interactions.
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| Peptide Synthesis |
To investigate whether macrophages are capable of internalizing biomolecules via HSPGs, they were incubated with the FITC-labeled cell-penetrating peptides penetratin (RQIKIWFQNRRMKWKK) and TAT (YGRKKRRQRRR) (both FITC-Ahx modified at N-terminus, >85% pure, GenScript). |
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