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Structural basis of pre-tRNA intron removal by human tRNA splicing endonuclease

Molecular Cell. 2023-04; 
Xiaofeng Zhang, Fenghua Yang, Xiechao Zhan , Tong Bian, Zhihan Xing, Yichen Lu, Yigong Shi
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Gene Synthesis The sequence of human pre-tRNAARG-UCU gene (NCBI reference: NC_000017.11) was synthesized by GenScript and fused downstream of a T7 promotor. Get A Quote

摘要

Removal of the intron from precursor-tRNA (pre-tRNA) is essential in all three kingdoms of life. In humans, this process is mediated by the tRNA splicing endonuclease (TSEN) comprising four subunits: TSEN2, TSEN15, TSEN34, and TSEN54. Here, we report the cryo-EM structures of human TSEN bound to full-length pre-tRNA in the pre-catalytic and post-catalytic states at average resolutions of 2.94 and 2.88 Å, respectively. Human TSEN features an extended surface groove that holds the L-shaped pre-tRNA. The mature domain of pre-tRNA is recognized by conserved structural elements of TSEN34, TSEN54, and TSEN2. Such recognition orients the anticodon stem of pre-tRNA and places the 3'-splice site and 5'-splice site into... More

关键词

RNA metabolism; splicing mechanism; tRNA splicing; tRNA splicing endonuclease
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