Products/Services Used | Details | Operation |
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Codon Optimization> | 2A-EGFP cassette in PX461 was replaced with synthesized UGI-2AEGFP cassette (Genscript)./Human codon optimized deaminases LpCDA1, LpCDA1L1_1/3/4,LpCDA1L2_1/2, LjCDA1, LjCDA1L1_1/4, LjCDA1L2_1/2, PmCDA1L1_4 and PmCDA4 were synthesized commercially (Genscript) | Get A Quote |
Peptide Synthesis> | Get A Quote |
Base editing tools for cytosine to thymine (C-T) conversion enable genome manipulation at single base-pair resolution with high efficiency. Available base editors (BEs) for C-T conversion (CBEs) have restricted editing scopes and nonnegligible off-target effects, which limit their applications. Here, by screening diversified lamprey cytidine deaminases, we establish various CBEs with expanded and diversified editing scopes, which could be further refined by various fusing strategies, fusing at either N-terminus or C-terminus of nCas9. Furthermore, off-target analysis reveals that several CBEs display improved fidelity. Our study expands the toolkits for C-T conversion, serves as guidance for appropriate choice ... More