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Generation of RUNX1c-eGFP induced pluripotent stem cell, MUSIi012-A-4, using CRISPR/Cas9

Stem Cell Res. 2023-01;

Pimonwan Srisook, Chuti Laowtammathron, Chanchao Lorthongpanich, Phatchanat Klaihmon, Papussorn Terbto, Supaporn Waeteekul, Yaowalak U-Pratya, Surapol Issaragrisil

Products/Services Used	Details	Operation
Gene Synthesis	… CRISPR/Cas9 plasmid construct containing sgRNA targeting exon 1 of RUNX1c (GenScript Biotech) and the knock-in vector was used to generate the RUNX1c-eGFP reporter stem cell …	Get A Quote

摘要

Runt-Related Transcription Factor 1c (RUNX1c) plays an important role in regulating the development of hematopoietic stem cells (HSC). Using CRISPR/Cas9 gene editing technology, we established a RUNX1c-eGFP reporter cell line from the MUSIi012-A cell line. The MUSIi012-A-4 cell line has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro and in vivo. This cell line serves as a valuable model to observe the expression of RUNX1c via eGFP tracking during human hematopoietic development.