Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis> | … (pET28a-luciferase) were ordered from GenScript (Nanjing, China) with sequence listed in supplementary note 1. All chemicals and reagents used were analytical grade and prepared … | Get A Quote |
Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost. Here, we described a novel method, designated SENSOR (Sulfur DNA mediated nucleic acid sensing platform), for rapid nucleic acid detection. SENSOR was developed from phosphorothioate (PT)-DNA and sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. SENSOR utilizes PT-DNA oligo and SBD as targeting module, which is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested detection on synthesized nucleic acid and COVID-19 pseudovirus, a... More