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Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome

Plant Cell Rep. 2024-06; 
Lamiaa M Mahmoud, Manjul Dutt
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Proteins, Expression, Isolation and Analysis … New England Biolabs, Ipswich, MA, USA). We incubated a Cas9-EGFP fusion protein (GenCrisper Nls-Cas9-EGFP, GenScript, USA; Piscataway, NJ, USA) that was diluted in 10 × Cas9 … Get A Quote

摘要

A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

关键词

Albino phenotype, CRISPR/Cas9, Citrus, Genome editing, Marker-free, Precision breeding
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