• 描述
  GenCrispr NLS-Cas9-EGFP is a fusion protein developed by GenScript. It contains a nuclear localization signal (NLS) on its N terminal end, and an EGFP and a 6x(His) sequence on the C terminal end. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 protein forms a highly stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. When Cas9 is expressed with an NLS sequence, the Cas9 RNP complex can localize to the nucleus immediately upon entering the cell. There is no requirement for in vivo transcription or translation, which improves the efficiency of this method over plasmid-based systems. Additionally, the Cas9 RNP complex is rapidly cleared from the cells minimizing the chance of off-target cleavage when compared to other systems (Kim, et al. 2014). The EGFP tag can be used as a reporter for tracking or sorting transfected cells, which enables enrichment of cell populations for desired genome edits via fluorescence activated cell sorting (FACS). It significantly reduces the labor and cost associated with single cell cloning and genotyping in genome editing applications. 
• 图片:
  

  [4 Images]

• 抗原物种:
  Mouse
• 表达系统:
  HEK 293
• 蛋白结构:

  PD-L1 (Phe19-Thr238) Accession # Q9EP73	hFc
  N-term	C-term

• 同义词:
  CD274; B7-H; B7H1; PDCD1L1; PDCD1LG1; PDL1; CD274 molecule; Programmed cell death ligand 1; hPD-L1
• 图片:
  

  [1 Image]

18 Citations

• 浓度
  4-20%, gradient
• 描述
  GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness. SurePAGE gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis, increases gel stability and minimizes protein modification. SurePAGE gels guarantee excellent batch-to-batch consistency and a reliable protein migration pattern. With specially formulated Tris-MOPS running buffer, proteins can be separated quickly and efficiently for subsequent detection by staining or Western blotting. Bis-Tris, 4-20%, 12 wells Cassette size: 100 x 84.5 x 4.7 mm (L x W x T) Gel size: 80 x 70 x 1 mm (L x W x T)
• 图片:
  

  [3 Images]

11 Citations

• 浓度
  4-12%, gradient
• 描述
  GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness. SurePAGE gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis, increases gel stability and minimizes protein modification. SurePAGE gels guarantee excellent batch-to-batch consistency and a reliable protein migration pattern. With specially formulated Tris-MOPS running buffer, proteins can be separated quickly and efficiently for subsequent detection by staining or Western blotting. Bis-Tris, 4-12%, 10 wells Cassette size: 100 x 84.5 x 4.7 mm (L x W x T) Gel size: 80 x 70 x 1 mm (L x W x T)
• 图片:
  

  [3 Images]

4 Citations

• 浓度
  8-16%, gradient
• 描述
  GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness. SurePAGE gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis, increases gel stability and minimizes protein modification. SurePAGE gels guarantee excellent batch-to-batch consistency and a reliable protein migration pattern. With specially formulated Tris-MOPS running buffer, proteins can be separated quickly and efficiently for subsequent detection by staining or Western blotting. Bis-Tris, 8-16%, 15 wells Cassette size: 100 x 84.5 x 4.7 mm (L x W x T) Gel size: 80 x 70 x 1 mm (L x W x T)
• 图片:
  

  [3 Images]

11 Citations

• 浓度
  4-12%, gradient
• 描述
  GenScript SurePAGE, Bis-Tris, 10 cm x 8 cm gels are high-performance precast mini polyacrylamide gels with a special design that allows large sample loading volumes. The unique formulation of the gel and cassette design enables superior band resolution and significantly improved band evenness. SurePAGE gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis, increases gel stability and minimizes protein modification. SurePAGE gels guarantee excellent batch-to-batch consistency and a reliable protein migration pattern. With specially formulated Tris-MOPS running buffer, proteins can be separated quickly and efficiently for subsequent detection by staining or Western blotting. Bis-Tris, 4-12%, 15 wells Cassette size: 100 x 84.5 x 4.7 mm (L x W x T) Gel size: 80 x 70 x 1 mm (L x W x T)
• 图片:
  

  [3 Images]

2 Citations

• 抗原物种:
  Human
• 表达系统:
  CHO
• 蛋白结构:

  IL-4 (His25-Ser153) Accession # P05112

• 同义词:
  IL4; B cell growth factor-1; BCGF-1; B cell stimulatory factor-1; BSF-1; B cell differentiation factor; BCDF; T cell growth factor-2; TCGF-2; Mast-cell growth factor-2; MCGF-2
• 图片:
  

  [3 Images]

• 抗原物种:
  Human
• 表达系统:
  CHO
• 蛋白结构:

  IL-5 (Ile20-Ser134) Accession # P05113

• 同义词:
  IL5; EDF; BCDFII; TRF
• 图片:
  

  [2 Images]

• 抗原物种:
  Human
• 表达系统:
  E. coli
• 蛋白结构:

  MIF (Met1-Ala115) Accession # P14174

• 同义词:
  Macrophage Migration; Inhibitory Factor; GLIF; MMIF; GIF; Glycosylation-inhibiting factor
• 图片:
  

  [1 Image]

• 抗原物种:
  Rat
• 表达系统:
  E. coli
• 蛋白结构:

  IL-3β (Met26-Cys169) Accession # P97688

• 同义词:
  Hematopoietic Growth Factor; MCGF; Multipotential Colony-stimulating Factor; P-cell-stimulating Factor