Human

HEK 293

PVR/CD155 (Trp21-Asn343) Accession # P15151	hFc
N-term	C-term

CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR

GenCrispr Cas9-C-NLS nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein with a C-terminal nucleic localization signal (NLS) that can be used for genome editing by inducing site-specific DNA double stranded breaks. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system, which can localize to the nucleus immediately once entering the cell with the guide of the NLS. Compared with the mRNA or plasmid systems, transcription and translation processes are not required. This DNA-free system avoids the risk of inserting foreign DNA into the genome, which can be quite useful for gene editing-based disease therapy. Our highly pure and active Cas9 nuclease meets all of the researcher’s requirements (e.g. in vitro cleavage assay, RNP complex transfection, micro injection). Product Source: GenCrispr Cas9-C-NLS is produced by expression in an E. coli strain carrying a plasmid encoding the Cas9 gene from Streptococcus pyogenes with a C terminal nuclear localization signal (NLS). Key features: DNA-free: no external DNA added to system. High cleavage efficiency: NLS ensures the entry of Cas9 protein into nuclei. Low off target: transient expression of Cas9 nuclease. Time-saving: no need for transcription and translation. With this Cas9 Nuclease, you can: Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage. In vivo gene editing combined with specific gRNA by electroporation or injection.

Human

Neuromedin U receptor 1 (NMUR1) is a member of G-protein coupled receptor family A and is the most abundant in peripheral tissue, particularly in small intestines and stomach. This protein is a receptor for Neuromedin U (NMU-25). The first biological activity ascribed to NmU was smooth muscle contraction. Neuromedin U has also been reported to increase arterial blood pressure and modify ion transport in the intestinal tract. Finally, NmU injected subcutaneously into rats has been reported to result in a short term increase in circulating ACTH levels and a long term increase in serum corticosterone levels, suggesting a role in regulation of the hypothalamo-pituitary-adrenal axis.

T7 Endonuclease I (T7E1) recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, hetero duplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5´ to the mismatch. The protein is the product of T7 gene 3. GenCrispr T7 Endonuclease I is a fusion protein produced from E.coli.

Human

Somatostatin receptors (SSTRs), a family of seven transmembrane (TM) domain G-protein-coupled receptors having five distinct subtypes (termed SSTR1–5), are activated by somatostatin secreted from the nerve and endocrine cells. SSTRs are widely expressed in many tissues, frequently as multiple subtypes that coexist in the same cell. With expressions in a tissue-specific manner, SSTRs are involved in the regulation of secretion of insulin, glucagon and growth hormone as well as cell growth induced by neuronal excitation in both the central and peripheral nervous systems. The five receptors share common signaling pathways such as the inhibition of adenylyl cyclase, activation of phosphotyrosine phosphatase (PTP), and modulation of mitogen-activated protein kinase (MAPK) through G-protein-dependent mechanisms. Aberrant expression of somatostatin receptors is known to be involved in a large number of human tumors. The human medullary thyroid carcinoma cell line TT expresses all SSTR subtypes. SSTR5 induces cell cycle arrest via PTP-dependent modulation of MAPK, which is associated with the induction of the retinoblastoma tumor suppressor protein and p21. In addition, SSTR 5 displays acute desensitization of adenylyl cyclase coupling and undergoes rapid agonist-dependent endocytosis.

eStain 清洗试剂盒是专门为 eStain L1/LG 蛋白染色仪开发的一款清洗液试剂盒，试剂盒 包含 2 瓶 500ml 清洗母液和一个 5L 空桶，清洗液母液为无色或淡黄色液体，定期使用此试剂 清洗 eStain L1/LG 染色设备，可以有效清除仪器通道内沉积的杂质，降低染色背景，延长设 备使用寿命。本试剂处于室温阴凉干燥处可保存 1 年。

Other applications;

Rabbit IgG

Unconjugated

Not applicable

GenScript Western Quick Block Kit and Western Quick Block Optimization Kit are for quick blocking of any membranes used for Dot blot and Western blot. It can also be used for quick blocking of any solid surface such as microtiter plates used for ELISA. Instead of classical one-hour blocking of the solid surface with 5–10% dry milk, this kit enables the complete blocking of the solid surface in just 5 minutes. Furthermore, Western Quick Block Kit significantly increases the Western detection sensitivity compared with dry milk blocking. And less amount of antibodies can be used to reach the same antigen detection level as using classical blocking method.

Human

GPR109A is a high affinity receptor for nicotinic acid (niacin) and is a member of the nicotinic acid receptor family of G protein-coupled receptors (the other identified member being GPR109B). GPR109A is a Gi/o protein-coupled receptor with high affinity for nicotinic acid. In GPR109A knockout mice, the effects of niacin on both lipids and flushing are eliminated. Furthermore in arrestin beta 1 knockout mice, niacin's effect on flushing is greatly reduced while the lipid modifying effects are maintained. GPR109A is believed to be an important biomolecular target of niacin which is a widely prescribed drug for the treatment of dyslipidemia and to increase HDL cholesterol but whose therapeutic use is limited by flushing.

Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Equilibration Buffer (10X ) and 2x 15pK eBlot L1 Transfer Sponge. The kit is for 30x transfers with standard program.