A GPI Signal Peptide-Anchored Split-Ubiquitin (GPS) System for Detecting Soluble Bait Protein Interactions at the Membrane.
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Operation |
| Peptide Synthesis |
peptide sequence (Fig. 1B) in front of the Gateway cassette of the bait vector pMetOYC-Dest of the CytoSUS system (Karnik et al., 2015) using unique restriction sites XhoI and XbaI (GenScript USA; HindIII also was introduced for future cloning work), replacing a sequence encoding an OST4 leader, to obtain the vector pExg2Met-Dest for the Exg2 GPI signal peptide-anchored mbSUS (GPS) system (Fig. 1C; see Supplemental Fig. |
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