Resistance to inhibitors of cholinesterase-8A (Ric-8A) and Ric-8B are essential biosynthetic chaperones for heterotrimeric G protein α subunits. We provide evidence for the direct regulation of Ric-8A cellular activity by dual phosphorylation. Using proteomics， Western blotting， and mutational analyses， we determined that Ric-8A was constitutively phosphorylated at five serines and threonines by the protein kinase CK2. Phosphorylation of Ser and Thr in rat Ric-8A (corresponding to Ser and Thr in human Ric-8A) was required for high-affinity binding to Gα subunits， efficient stimulation of Gα subunit guanine nucleotide exchange， and mediation of Gα subunit folding. The CK2 consensus sites that contain Ser and Thr are conserved in Ric-8 homologs from worms to mammals. We found that the homologous residues in mouse Ric-8B， Ser and Ser， were also phosphorylated. Mutation of the genomic copy of in to encode alanine in the homologous sites resulted in characteristic reduction-of-function phenotypes that are associated with defective G and G signaling， including reduced locomotion and defective egg laying. The phosphorylation site mutant phenotypes were partially rescued by chemical stimulation of G signaling. These results indicate that dual phosphorylation represents a critical form of conserved Ric-8 regulation and demonstrate that Ric-8 proteins are needed for effective Gα signaling. The position of the CK2-phosphorylated sites within a structural model of Ric-8A reveals that these sites contribute to a key acidic and negatively charged surface that may be important for its interactions with Gα subunits.