Transcription factors of Schizophyllum commune involved in mushroom formation and modulation of vegetative growth
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The 1832 bp HindIII/SbfI fragment of pHom2 containing hom2 coding and flanking sequences was cloned in pSP72 using the same sites, resulting in pHom2HF. In the next step, a 692 hom2 coding fragment was synthesized (Genscript, Piscataway, NJ, USA), in which the serine codons in the 4 RRXS motifs were replaced for alanine (hom2con). |
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