Histone acetylation is an important post-translational modification in eukaryotes and is regulated by two antagonistic enzymes， namely histone acetyltransferase (HAT) and histone deacetylase (HDAC). However， little has been done on the HAT superfamily in Brachypodium distachyon (B. distachyon)， a new model plant of Poaceae. In this study， eight HATs were identified from B. distachyon and classified into four major families. Subcellular localization analysis showed that a majority of BdHATs were predominantly localized in the nucleus. Syntenic and phylogenetic analysis indicated there may be two common ancestral CREB-binding protein (p300/CBP， HAC) genes prior to the separation of monocots and dicots. Expression analysis revealed that the potential roles of BdHATs in B. distachyon development and responses to four abiotic stresses. Protein-protein network analysis identified some potential interactive genes with BdHATs. Thus， our results will provide solid basis for further study the function of HAT genes in B. distachyon and other monocot plants.