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Catalog Antibody> | … Cells were fixed using ice cold ethanol: ace- tic acid (95:5) for 10 min. Cells were permeabilized using 0.1% Triton-X100. Cells were incubated with anti pdmH1N1 HA antibody (GenScript, USA) (1:1,000) followed by FITC-conjugated anti-rabbit IgG (Sigma, USA) (1:500 in PBS) … | Get A Quote |
Chromatin fibers within the interior of the nucleus of the cell make stable interactions with the nucleoskeleton, an ensemble of ``extra-chromatin'' structures which help ensuring genome stability. Although the role of these interactions appears crucial to the correct behavior of the cell, their impact on chromatin structure and dynamics remains to be elucidated. In order to tackle this important issue, in this work we introduce a simple polymer model for chromatin fibers in interphase which takes into account the two generic properties of chain-vs.-chain mutual uncrossability and the presence of stable binding interactions to an extra-chromatin nuclear matrix. To study how these constraints affect chroma... More