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Rational Design， Structure-Activity Relationship， and Immunogenicity of Hypoallergenic Pru p 3 Variants.

Mol Nutr Food Res. 2019-06;

EichhornStephanie，HörschlägerAngelika，SteinerMarkus，LaimerJosef，JensenBettina M，VersteegSerge A，PablosIsabel，BrizaPeter，JongejanLaurian，RigbyNeil，AsturiasJuan A，PortolésAntonio，Fernandez-RivasMontserrat，PapadopoulosNikolaos G，MariAdriano，PoulsenLars K，LacknerPeter，van ReeRonald，FerreiraFatima，GadermaierGabr

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Peptide Synthesis	… The endotoxins of the recombinant Bet v 1 polypeptide preparations were removed by the Detoxi-Gel Endotoxin Removing Gel (Thermo: Cat. No. 20344) where after endotoxin content was analysed by ToxinSensor Endotoxin Detection System (GenScript: Cat. No. L00350C) …	Get A Quote

摘要

Allergies to lipid transfer proteins involve severe adverse reactions; thus， effective and sustainable therapies are desired. Previous attempts disrupting disulfide bonds failed to maintain immunogenicity; thus， the aim is to design novel hypoallergenic Pru p 3 variants and evaluate the applicability for treatment of peach allergy.