| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | Alexa Fluor-conjugated secondary antibodies were pur- Spleens isolated from OT-I mice were disrupted through 70 μm cell strainers (BD Biosciences), washed once and, for stimula- tion, resuspended in CTL medium containing Iscove’s Modified Dulbecco’s Media (Invitrogen), 10% FCS, 2 mM L-glutamine, 50 μM β-mercaptoethanol, 50 U/mL penicillin and streptomycin, 100 U/mL Proleukin IL-2 (Novartis), and 10 nM OVA257-264 pep- tide [SIINFEKL] (GenScript). | Get A Quote |
Cytotoxic T lymphocytes (CTLs) kill tumorigenic and virally infected cells by targeted secretion of lytic granule contents. The precise point at which secretion occurs is directed by the centrosome docking at the immunological synapse (IS). The centrosome is highly dynamic in CTLs, lagging behind the nucleus in the uropod of migrating CTLs, but translocating across the entire length of the cell to dock at the IS when a target cell is recognized. While in most cell types, the centrosome is always closely associated with the nuclear membrane, in CTLs, it often appears to be dissociated from the nucleus, both in migrating cells and when forming an IS. We asked whether this dissociation is required for CTL killing,... More
