| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | The quantity of IFN-γ-secreting T cells in the peripheral blood during this trial was assessed by ELISpot using the Monkey IFN-γ kit (3420 M-2HPW-2, MABTECH Inc., Cincinnati, OH, USA) following the manufacturer’s instructions [19, 20]. The peptides used in this study (Table 2) were previously identified as showing reactivity in a pilot experiment with cynomolgus monkeys, and were synthesized by Genscript Biological Technology Co., Ltd. (Nanjing, China). For each library, peptides were dissolved in 100% dimethyl sulfoxide (DMSO, Sigma) and pooled at a concentration of 2 mg/mL per peptide, and each pool was tested at a final concentration of 5 μg/mL per peptide in the ELISpot assays. Three peptide pools were used as stimulatory agents. Hence, the ELISpot assays were carried out in five groups, including the negative control (unrelated peptide in DMSO, DLMDLMGYIPLV), core pool, Env pool, Pol pool, and positive control (anti-CD3 IgG, 1:1000, provided in the kit). | Get A Quote |
Background A new promising therapeutic approach has emerged for patients chronically infected by the hepatitis B virus (HBV) with the development of a non-replicative adenovirus vector vaccine candidate (Ad-HBV). The vaccine encodes a fusion protein composed of a truncated HBV core protein, mutated polymerase protein, and two envelope domains. In this study, we assessed the immunogenicity of Ad-HBV administered to cynomolgus monkeys during a non-clinical safety assessment. Methods The virus was subcutaneously administered at 1.0 × 109 viral particles (VP)/animal (low-dose group), 1.0 × 1010 VP/animal (mid-dose group), and 1.0 × 1011 VP/animal (high-dose group); the control groups were administer... More
