GenScript). ... 3.1 Construction of the Expression Vector, pVJGFPa. 1. Order a synthetic gene (usually supplied in a plasmid) containing a His 6 -tag, the 26–47 flagellin segment of S. typhimurium and an EK cleavage site (GenScript). ...">
Products/Services Used | Details | Operation |
---|
The flagellum-specific export system is a specialized type III export machinery, which exports external flagellar proteins through the central channel of the flagellar filament. A number of evidence indicates that short segments within the disordered N-terminal region of flagellar axial proteins are recognized by the flagellum-specific export apparatus. Recently, we have demonstrated that the 26-47 segment of Salmonella typhimurium flagellin is capable of mediating flagellar export. N-terminal flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9-40 kDa) facilitating their secreted expression and easy pur... More