GenScript). ... 3.1 Construction of the Expression Vector, pVJGFPa. 1. Order a synthetic gene (usually supplied in a plasmid) containing a His 6 -tag, the 26–47 flagellin segment of S. typhimurium and an EK cleavage site (GenScript). ...">

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The Use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella.

Methods Mol Biol.. 2012-06;  824:131-143
Vonderviszt F, SajÓ R, DobÓ J, ZÁvodszky P. Research Institute of Chemical and Process Engineering, Faculty of Information Technology, University of Pannonia, VeszprÉm, Hungary
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摘要

The flagellum-specific export system is a specialized type III export machinery, which exports external flagellar proteins through the central channel of the flagellar filament. A number of evidence indicates that short segments within the disordered N-terminal region of flagellar axial proteins are recognized by the flagellum-specific export apparatus. Recently, we have demonstrated that the 26-47 segment of Salmonella typhimurium flagellin is capable of mediating flagellar export. N-terminal flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9-40 kDa) facilitating their secreted expression and easy pur... More

关键词

Flagellum-specific export; Type III secretion; Export signal; Recombinant protein; Salmonella typhimurium; Secreted over-expression; Flagellin
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