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Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors

Pract Lab Med. 2021-04; 
Zsófia Szabó, Tamás Szabó, Kornélia Bodó, Gábor Kemenesi, Fanni Földes, Katalin Kristóf, Eszter Barabás, Barna Vásárhelyi, Zoltán Prohászka, Eszter Fodor, Ferenc Jakab, Timea Berki, Zsombor Lacza
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Gene Synthesis … descriptions. Generic Assay (GA) and GenScript ELISA tests were performed at Semmelweis University, Department of Laboratory Medicine. GA assay (Cat No … positive). The GenScript Surrogate Virus Neutralization Test Kit (Cat No … Get A Quote

摘要

Serological testing is a tool to predict protection against later infection. This potential heavily relies on antibody levels showing acceptable agreement with gold standard virus neutralization tests. The aim of our study was to investigate diagnostic value of the available serological tests in terms of predicting virus neutralizing activity of serum samples drawn 5-7 weeks after onset of symptoms from 101 donors with a history of COVID-19. Immune responses against Receptor Binding Domain (RBD), Spike1 and 2 proteins and Nucleocapsid antigens were measured by various ELISA tests. Neutralizing antibody activity in serum samples was assessed by a cell-based virus neutralization test. Spearman correlation coeffic... More

关键词

AUC, area under the curve, Antibody response, COVID-19, COVID-19, coronavirus disease 2019, Correlate of protection, ECDC, European Centre for Disease Prevention and Control, NAbs, neutralizing antibodies, NC, Nucleocapsid, Neutralization, OD, optical density, ROC, receiver operating characteristic, S1, Spike protein 1, S2, Spike protein 2, SARS-CoV-2, SARS-CoV-2, Severe acute respiratory syndrome coronavirus 2, Serological test, VNT, virus neutralization test
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