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Optimization of Fermentation Conditions for Reteplase Expression by Using Response Surface Methodology.

Avicenna J Med Biotechnol. 2016-03; 
ZareHamze，Mir Mohammad SadeghiHamid，AkbariVa
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Codon Optimization … efficiency and mRNA stability. Software is available to design an optimized sequence for expression in a particular host (OPTIMIZER, Puigbo et al. 2007 or OptimumGene from Genscript). Several companies offer the possibility … Get A Quote

摘要

Expression of heterologous proteins at large scale is often a challenging job due to plasmid instability， accumulation of acetate and oxidative damage in bioreactors. Therefore， it is necessary to optimize parameters influencing cell growth and expression of recombinant protein.

关键词

Bioreactors，Escherichia coli，Fermentation，Retep
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